Study of Antioxidant Activity in Annona Leaves

Abstract

Abstract
This study aims to test the antioxidant activity of extracts of the leaves of the
Annona squamosal linn plant, which is commonly used in folk medicine and
is considered one of the most important anticancer agents at the present time.
The DPPH reagent inhibition method was utilized to estimate the total
antioxidants, (where (DPPH) is 2,2- diphenyl-1- picrylhydrazyl) revealing
that the extract concentrations required for 50% inhibition of free radicals
(IC50) was 3766.14μg/ml. The antioxidant equivalent to ascorbic acid was
3823.22μg/ml, which was the highest efficacy for a concentration of 5000
mg/ml of the extract and the lowest at a concentration of 1000mg/ml. This
study also assessed the contents of phenolics for their in vitro antioxidant
activity. The total phenolic content of methanol extract of Annona squamosal
linn measured by Folin-Ciocalteau reagent in terms of gallic acid ranged
from 1189.75 to 3502.25 mg GAE/g of extract , (where(GAE) is gallic acid
equivalent). The extract was tested against the following antibacterial types:
Staphylococcus aureus, Streptococcus spp, Escherichia coli, Klebsiella spp,
Psuedomones spp, and Protues spp. According to the findings, different
extract concentrations effectively inhibited the growth of the chosen
pathogenic microorganisms. The study revealed that the Annona squamosal
linn leaves contain large amounts of phenolic compounds which were found
to be the main contributor to its antioxidant activities, and the extract was
found to exert low to moderate antibacterial activity. Hence, it can be
concluded that the leaves of Annona squamosal linn can lead to the creation
of compounds that can be used to develop new and more effective anticancer
drugs.