The effect of Androctonus australis scorpion venom on human O blood group in vitro

Abstract

This study aims to determine the effect of Androctonus australisus scorpion venom on the O-type of human blood in-vitro. The number of study samples was 28, with 14 male samples (7 O+ and 7 O−) and 14 female samples (7 O+ and 7 O−). With 40 μL of each blood type mixed with 10 μL of venom without dilution, which was collected from 70 scorpions by electrical stimulation. The complete blood count (CBC) was analyzed before and after the venom was treated, and the results were compared. further, a blood smear was performed before and after the venom was treated. The results of CBC showed that there were moral differences (P < 0.05) in the average count of red blood cells and hemoglobin, but did not show moral differences (P > 0.05) in the average count of white blood cells and platelets after treatment with venom without taking into account gender and Rh factors, and when RH have been taken into account, the results showed that there were no moral differences (P > 0.05) in the average count of red and white blood cells, hemoglobin and platelets after treatment with venom, While taking the gender factor into account, the results showed that there were moral differences (P < 0.05) in the average count of red blood cells and hemoglobin after venom treatment, where female blood samples were more affected by venom than males, but did not show moral differences (P > 0.05) attributable to the gender factor in the average count of white blood cells and the platelets after the venom. The blood smear of the blood sample mixed with the venom showed deformation and hemolysis of red blood cells.