Study of Antioxidant Activity in Annona Leaves

Abstract

This study aims to test the antioxidant activity of extracts of the leaves of the Annona squamosal linn plant, which is commonly used in folk medicine and is considered one of the most important anticancer agents at the present time. The DPPH reagent inhibition method was utilized to estimate the total antioxidants, (where (DPPH) is 2,2- diphenyl-1- picrylhydrazyl) revealing that the extract concentrations required for 50% inhibition of free radicals (IC50) was 3766.14μg/ml. The antioxidant equivalent to ascorbic acid was 3823.22μg/ml, which was the highest efficacy for a concentration of 5000 mg/ml of the extract and the lowest at a concentration of 1000mg/ml. This study also assessed the contents of phenolics for their in vitro antioxidant activity. The total phenolic content of methanol extract of Annona squamosal linn measured by Folin-Ciocalteau reagent in terms of gallic acid ranged from 1189.75 to 3502.25 mg GAE/g of extract , (where(GAE) is gallic acid equivalent). The extract was tested against the following antibacterial types: Staphylococcus aureus, Streptococcus spp, Escherichia coli, Klebsiella spp, Psuedomones spp, and Protues spp. According to the findings, different extract concentrations effectively inhibited the growth of the chosen pathogenic microorganisms. The study revealed that the Annona squamosal linn leaves contain large amounts of phenolic compounds which were found to be the main contributor to its antioxidant activities, and the extract was found to exert low to moderate antibacterial activity. Hence, it can be concluded that the leaves of Annona squamosal linn can lead to the creation of compounds that can be used to develop new and more effective anticancer drugs.