STUDYING THE PREVALENCE OF QUINOLONES AND AMINOGLYCOSIDES RESISTANCE PROFILES AND TRANSMISSION OF AAC (6')-IB GENE FROM CHICKEN TO CONTACT HUMAN

Gamal A. Salem; Suzan K. Murad; Fathi B. Alamyel; Manal A. Ebowah; Marwa A. Mahmou; Abdullah R. Abulifa

doi:10.36602/mmsj/2016.n05.02

Authors

Gamal A. Salem Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
Suzan K. Murad Department of Microbiology, Faculty of Nursing, Misurata University, Misurata, Libya
Fathi B. Alamyel Department of Pharmacologyand Toxicology, Faculty of Pharmacy, Misurata University, Misurata, Libya
Manal A. Ebowah Faculty of Pharmacy, Misurata University, Misurata, Libya
Marwa A. Mahmou Faculty of Pharmacy, Misurata University, Misurata, Libya
Abdullah R. Abulifa Faculty of Chemistry, Faculty of Pharmacy, Misurata University, Libya

DOI:

https://doi.org/10.36602/mmsj/2016.n05.02

Keywords:

Antibiotic resistance, Quinolones, Aminoglycosides,, Enterobacteriaceae

Abstract

The spread of resistant Enterobacteriaceae to antibiotics in chicken farms constitute a reservoir of resistant genes
that could be easily transmitted to contact human. So, this study conducted to investigate the prevalence of ciprofloxacin and gentamycin resistance profiles and the frequency of aac (6')-Ib gene in chicken farms in Misurata city
and living population in the vicinity of these farms. (135) cloacae swab from chicken and (107) urine samples from
human living in these farms were collected. Isolation and identification of different Enterobacteriaceae strains is
performed in antibiotic resistance profiles against ciprofloxacin and gentamycin antibiotics. DNA extracted from
each sample and further screening for aac (6')-Ib-cr in ciprofloxacin and/or gentamycin resistant isolates by using of
using PCR technique. Our results showed that 88.8% and 93.4% of chicken and human isolates confirmed to be Enterobacteriaceae respectively. There were non-significant association between the source of Enterobacteriaceae isolates and quinolones or aminoglycosides resistance profiles. The screening of the genetic determinant of gentamycin
and ciprofloxacin resistance aac (6')-Ib-cr, revealed an impressive proximity between the frequency of this gene
within the chicken and human isolates (33.6%, 33 %) respectively. In conclusion quinolones and aminoglycosides
resistance profiles and the frequency of aac (6')-Ib-cr between chicken and human were nearly comparable, that
would suggest the possible transmission of this gene from chicken to human