The aim of the present study was to identify various bioactive constituents present in non-polar hexane extract of aerial part extract of Pergularia Tomentosa, of Chenopodiaceae family using GC-MS technique. The results showed presence of wide variety of constituents, mostly of them were nonpolar including long chain saturated and unsaturated hydrocarbons and their oxygenated derivatives in addition to many alkyl halides. Fatty acids were also found in the extract, in addition to some pentacyclic triterpenes, especially, Lupeol and Sitetosterol. The components existed exhibit reported diverse biological activity including antifungal, antibacterial, antioxidant, anticancer, anti-inflammatory and other activities.