The use of herbal medicinal products has recently increased because of bacterial resistance to conventional antibiotics. Plantago lanceolata and Helichrysum stoechas used in traditional medicine due to their medicinal properties. This current study aimed to perform perform the in vitro antibacterial activity of the extracts obtained from the fresh leaves of P. lanceolata and dry H. stoechas against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa isolated from patients by studying the inhibition of the growth of the pathogen E. coli and P. aeruginosa. The antibacterial activity of the ethanolic and aqueous decoction extracts of the plants was examined. The results indicated that there was no antibacterial activity of P. lanceolata and H. stoechas observed against Gram-negative bacteria used in this study. Therefore, further investigations and future experiments need to be carried out to determine the effect of these herbal extracts on other types of bacteria.