Fungi are usually found in indoor environments and cause many diseases. The aim of the study was to evaluate the level of airborne fungal contamination in various hospital rooms in the following centers: Tripoli University Hospital, Diabetes Clinic - Tripoli, and Metiga Military. Medical Reference Laboratory Hospital - Tripoli, Hospital Dermatology Beer Osta Milad . A total of 100 air samples were conducted from five hospital environments. By screening 100 petri dishes containing sabouraud dextrose agar medium, Determine the places where dishes are displayed in these public hospitals, in general examination rooms, emergency rooms, etc., by removing the covers from the dishes for 15 minutes. The dishes were then placed at room temperature for a period of 72-120 hours. Through virtual examination of these dishes, it was revealed that there was dense growth covering the dishes. Microscopic examination proved that atmospheric air can be considered a repository of fungal germs and spores, and through the results obtained Many genera of fungi (8 genera) have been isolated, including opportunistic pathogenic fungi such as Candida spp, fast-growing, spore-producing fungi such as Aspergillus spp, and others. The most frequently isolated fungi include the genera Penicillium spp (29.6%), Alternaria spp (19.4%), Streptomyces spp (19.4%), Aspergillus spp (15.3%), Candida spp (9.2%), and Sterilia mycelia spp ( 4,1%, Ulocladium spp (2,0%) and Microsporum canis (1,0%)