تقدير الفيتامينات B1, B2, B9 في بعض عينات الخضار: الكرنب والبنجر والقرنبيط والخس بطريقة كروماتوجرافيا السائل ذات الكفاءة العالية

The contents of B1,B2 and B9 were estimated in some vegetable samples including: (Cabbage, Beets, Cauliflower and Lettuce) by HPLC technique. The concentration-swere measured from the calibration cueve also the validation methods were calculated. The evaluation of calibration model was based on the coefficient of determination (R2).The linearity of the concentrations was studied according to values of slope and intercept of the regression line and the correlation coefficient (R2).The fruitful linearity was obtained for the compounds between the concentrations and peak area , where the concentration which gave good linearity were ranged as following: 25 – 200 μg/ml ,5 – 25 μg/ml and 100 – 400 μg/ml for B1 , B2 and B9 , respectively.