Printing ISSN 2519-9749
Antimicrobial activity of the crude ethanol extracts of punica granatum L and Oregano. vulgare L
against multidrug resistant UTi isolates were investigated. Two strains of Escherichia coli and one
Acinetobacter that showed resistance against maximum number of tested antibiotic were selecte for an
antibacterial plant extract assy. Double –disk synergy (DDS) test was use for detection of Extended
Spectrum Bets-Lactamese (ESBLs) in the three studied UTi isolates. Antibacterial activities of the two
plants extract were measure by well diffusion, CUF/ml, and turbidity (O.D595) methods before and
after treatment. The results showed that punica granatumL peels extract have a significant
antimicrobial activity against the three tested UTi isolates. Diameters of inhibition zone were 25mm,
23mm, cuf/ml 6.6x 10-1 2.0 x 10-1
, O.D595 0.2 and 0.219 for Escherichia coli and Acinetobacter
sequentially compare with control. Antibacterial activity of ethanol extract was depended on plants
extract concentration and bacterial type. This study showed that plant extracts have negative impact on
the level of protein in treated bacterial isolates comparing to the control