Printing ISSN 2519-9749
Extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae in animals has a major
concern due to the possible transmission between them and human that may cause a public health threat. Our
study is conducted to estimate the prevalence of resistance pattern and characterization of ESBL producibility
and genes in Enterobacteriaceae isolated from chicken feces from poultry farms in Misurata city. 135 fecal
samples from 45 chicken farms were collected. Isolation and identification of different
Enterobacteriaceae strains were performed to these isolates. In addition, Antibiotic Resistance Profile (ARP)
against β-lactam antibiotics, ESBL phenotypic screening and PCR for ESBL genes; CTX, SHV and TEM,
were done. The results showed that 88.8 of chicken isolates confirmed to be Enterobacteriaceae. 45% of
chicken Enterobacteriaceae isolates were proved to be ESBL producing. The frequency of ESBL genes
showed that multigene producers were 66.7% (36/54). CTX gene was detected in 47 (87%) isolates and TEM
gene accounts for 39 (72.2 %) isolates, while the least proportion was for SHV 20 (37 %) isolates. From
previous study we can conclude that the prevalence of ESBL Enterobacteriaceae and their genes in poultry
farms of Misurata city would account for economic and public health threat in the society