Diabetic foot is a general health problem for diabetics, and identifying the bacteria causing it is a major role to know the type of appropriate treatment with antibiotics, The injury results from many complications such as nerve damage and lack of blood ischemia, The study was conducted on 40 diabetes patients with diabetes mellitus visiting the outpatient clinic of Misurata Medical Center, The percentage of males 70% and females 30%, aged between 30 and 82 years, isolated 63 bacterial isolation, positive bacteria accounted for 49% and negative bacteria 51%. Staphylococcus aureus (30%), Staphylococcus epidermidis (17%) and Gamma Streptococci (2%) were identified as isolated Gram-positive bacteria, And Gram-positive bacteria were as follows: Pseudomonas aeruginosa (17%), Klebsiella spp and Proteus spp at 8% each, Enterobacter spp (5%), 6% Serratia spp (5%) Providencia stuartii, Morganella morganii (2%). The proportion of patients with polymicrobial infection was 52%, while the proportion of patients with a monomicrobial infection was 48%. The resistant bacteria were identified as MRSA (30%), Pseudomonas aeruginosa (beta-lactamase) (3%) of gram-negative bacteria, The aim of the study was to identify the bacteria causing the bacterial infection isolated from the diabetic foot, as well as the knowledge of bacteria resistant to multidrug antibiotics, whether the bacteria were separated from the wide-spectrum beta-lactase or methicillin-resistant bacteria. These procedures give the doctor the choice of appropriate antibiotics in the treatment and prevent the spread of bacteria resistant to multidrug antibiotics.