This study was done to evaluate the incidence of isolation of Proteus spp among clinical samples and to know its susceptibility pattern in Toproq Medical Center hospital. Total of 83 clinical isolates of Proteus spp were collected from wound, pus, sputum, urine, ear, skin and EET. OPD was recorded the most department that collected the specimens 53 (64). All the samples were inoculated onto nutrient agar, blood agar, MacConkey agar and incubated at 37co, overnight. Urine (53) and pus (31), the predominant sample of isolation. Female (52) were commonly affected than male (48). Proteus spp showed sensitivity to F 12 (14) followed by Nalidixic acid and Amoxicillin clavulinic acid (13) equally and found to have high antimicrobial resistance against of Ampicillin 48 (58) Gentamycin 40 (48). The high antibiotic resistance of Proteus spp may be a signal of unselective ingestion of antibiotics.31 which is very common in developing countries.