Petroleum hydrocarbon are considered to be the most common group of persistent organic contaminants and are known to be toxic to many organism. Bacteria involve in degradation of oil during which the complex toxic substance were detoxifying by the production of biosur-factant. The ability of bacteria to degrade hydrocarbons and survive is attributing to devolvement resistance by mutation. Therefore, specific gene was responsible for production of biosurfactant and degradation process. In this study, 57 Klebsilla pneumonia isolates obtained from 64 oil contaminated soil samples from automobile workshop and petrol station. Growth profile of the isolates culture in carbon free media supplemented by different carbon source (crude oil, benzene, engine oil) confirm there their biodegradation activity and potential. Klebsilla pneumonia isolate selected on the highest degrade clear zone on ager well diffusion method was further more subjected to gravimetric analysis to determine the degrading capacity of tested hydrocarbon which was 43 of benzene , 78 of crude oil,and75 of engine oil. Emulsification indexes (E24) were 68, 86,and 84 for benzene, crude oil and engine oil respectively. Through curing experiment, the isolate showed genetically stable, chromosomal mediated biodegradation activity. Hence, this strain can clean oil-polluted sites.