The acetophenone oxime was obtained in moderate yield (31%) throughout a reaction between the acetophenone and the hydroxylamine hydrochloride under basic conditions. The structural formula of the obtained acetophenone oxime was confirmed by the IR, NMR and mass spectrometer. The 1HNMR data also revealed that the acetophenone oxime was resulted in two geometrical isomeric forms, E- and Z- isomers, in the ratio of (8:1) respectively