Detection of Typical and Blast Cells in Acute and Chronic Leukaemia Using the Manual Method and Hematology Analyzer BC-720

Abstract

Introduction: Diagnosing Leukaemia requires precise identification of atypical white
blood cells. This research aims to compare the effectiveness of the BC-720 analyzer
and manual microscopy in identifying blast and typical cells in both acute and chronic
Leukaemia. Manual microscopy and the BC-720 analyzer were used to investigate
differential white blood cell counts from 68 Leukaemia patients and 18 controls.
Sensitivity and error rates for detecting abnormal cells were evaluated. The manual
approach had a recognition loss of 12%, but successfully identified 47.1% of cases. The
BC-720 identified 52.9% of cases, with higher detection rates in chronic patients
(41.2%) and control cases (5.9%). In acute cases, the detection loss was 8.0% lower.
Both methods showed advantages and limitations. The BC-720 demonstrated higher
detection rates for abnormal cells, particularly in acute Leukaemia, and lower
recognition losses compared to manual microscopy. It can aid in more accurate
Leukaemia classification and management.